solutions laminar flow hood Search Results


solutions laminar flow hood  (Labconco)
95
Labconco solutions laminar flow hood
Solutions Laminar Flow Hood, supplied by Labconco, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trizol reagent  (Thermo Fisher)
99
Thermo Fisher trizol reagent
Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solutions+laminar+flow+hood/TRIzol+REAGENT/pmc03934298-115-67-72
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leica dmi8 widefield microscope  (Danaher Inc)
99
Danaher Inc leica dmi8 widefield microscope
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
Leica Dmi8 Widefield Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solutions+laminar+flow+hood/DMi8+S+Inverted+Microscope+Solution/pmc09390741-204-5-5
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870-fl vertical laminar flow cabinet  (CRUMA Material de Laboratorio S.A)
90
CRUMA Material de Laboratorio S.A 870-fl vertical laminar flow cabinet
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
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under a laminar flow hood  (CRUMA Material de Laboratorio S.A)
90
CRUMA Material de Laboratorio S.A under a laminar flow hood
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
Under A Laminar Flow Hood, supplied by CRUMA Material de Laboratorio S.A, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laminar flow cabinet clean air es/fb  (Telstar Life Science)
90
Telstar Life Science laminar flow cabinet clean air es/fb
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
Laminar Flow Cabinet Clean Air Es/Fb, supplied by Telstar Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
laminar flow cabinet clean air es/fb - by Bioz Stars, 2026-09
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filtered-air laminar flow hood safeflow 1.2  (EuroClone)
90
EuroClone filtered-air laminar flow hood safeflow 1.2
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
Filtered Air Laminar Flow Hood Safeflow 1.2, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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class100 clean room  (Tamco Industries)
90
Tamco Industries class100 clean room
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
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class-100 laminar flow hood  (Bigneat Inc)
90
Bigneat Inc class-100 laminar flow hood
Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term <t>widefield</t> imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).
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Image Search Results


Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term widefield imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).

Journal: Cancer Discovery

Article Title: Pharmacologic Reduction of Mitochondrial Iron Triggers a Noncanonical BAX/BAK-Dependent Cell Death

doi: 10.1158/2159-8290.CD-21-0522

Figure Lengend Snippet: Ironomycin cell death is distinct from canonical apoptosis induced by BH3 mimetics. A, Confocal images of MCL1 −/− MEFs expressing tagged-OMM component TOMM20-Halo (JF646, red) with either IMM component TIMM23 (left; mNeonGreen, green) or MMX (right; tdTomato, green) after 24 hours untreated (top) or 3 μmol/L ironomycin (bottom). Insets highlight representative mitochondrial network morphology. B, Snapshots from long-term widefield imaging assay of MCL1 −/− Bax −/− Bak −/− MEFs reexpressing mNeonGreen-BAX and TOMM20-Halo, treated with 1 μmol/L ABT-737 + 20 μmol/L QVD-OPH or 500 nmol/L ironomycin, with insets highlighting BAX recruitment to mitochondria prior to cell death ( n = 3 independent imaging experiments). C, Scatter plot displaying the time until the appearance of BAX foci, quantified manually, from long-term widefield imaging assay. Each data point represents a single cell ( n = 3 biological replicates with >10 cells counted per experiment; means ± SD; **, P < 0.01; ***, P < 0.001). D, Fractionation experiment in MV4;11 cells treated with 500 nmol/L ironomycin showing cytochrome c (CYT C ) and BAX protein expressions by immunoblot in total cell, cytosolic fraction, and mitochondrial membrane fraction. VDAC1 is used as a loading marker of mitochondrial membrane fraction and HSP70 as a loading marker of cytoplasmic fraction ( n = 3; one representative experiment is shown). E, Cell viability assessed by FACS analysis of PI staining. We treated MV4;11 WT and MV4;11 cells with BCL2 overexpressed for 48 hours with ironomycin (top curve) and venetoclax (bottom curve, n = 3 biological replicates). F, Heat map showing the mRNA expression of BH3-only proteins in MV4;11 cells after 6 and 24 hours of ironomycin. G and H, Cell viability using PI FACS staining. We treated MV4;11 WT and MV4;11 cells with single knockout for the BH3-only proteins NOXA, PUMA, BIM, and BID ( G ) or double knockouts for BIM/BID and NOXA/PUMA ( H ) for 72 hours with ironomycin (top curves) and venetoclax (bottom curves; n = 3 biological replicates).

Article Snippet: Cells were imaged on a Leica DMi8 widefield microscope (within an environment-controlled chamber) using a 63×/1.3 NA glycerol immersion objective, with images captured at 12-minute intervals for 72 hours.

Techniques: Expressing, Imaging, Fractionation, Western Blot, Membrane, Marker, Staining, Knock-Out